Laboratory Research Technician III

The Pinzon Guzman is seeking a Research Technician III. The successful candidate will have prior basic science research experience. The Research Technician III will work closely with other lab members (such as Post Docs on high priority experiments. The Research Technician III will be required to perform moderately complex research activities while playing an important role in facilitating day-to-day operations of the lab. The Candidates will work on various projects such as: “Restoring the BMP/Noggin Balance in Esophageal Atresia: Integrating Pathogenesis with Surgical Innovation to Improve Outcome.”

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Responsibilities: Human cell culture (routine handling, transfection, flow cytometry) and other standard bench work like Western Blot. DNA isolation from cells, PCR amplification. Maintaining laboratory stocks of lab supplies, reagents, buffers, plates, etc. and order new supplies as needed. Performing routine maintenance of laboratory instruments. Assisting in analyzing and summarizing data for scientific communications (e.g., grant applications, peer-reviewed publications). Maintaining records of experimental data and communicating results to lab members. Reviewing pertinent scientific literature. Other duties as assigned.

Must have an understanding of basic molecular biology and genetics. Must have excellent verbal and written communication skills. Theoretical and practical knowledge of molecular cloning techniques is essential (DNA/RNA isolation, restriction digestion, PCR amplification, purification, etc.). Experience with Organoids. Experience with the proper handling and genetic manipulation of bacteria is essential. Proficiency with basic computational tools. Experience with flow cytometry and FACS would be an asset.

Physical effort associated with wet lab work, i.e. standing while conducting experiments, sitting while working on computer, walking back and forth in the lab to sinks and microscopes as well as occasionally going to the loading doc to pick up supplies, lifting packages less than 20lbs., etc.